Brian C.

Week 7 - 7/15/2013
Good results page Brian. - Dr. B 071713
HAPPY BIRTHDAY TO ME!

DNA Sequencing Results for last week's FabV plasmid are in:

Using PLic Forward:

NNNNNNNNGTNNACTTTANNNGAGANATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGATCTGGGTACCGAG
AACCTGTACTTCCAATCCATGATTATCAAGCCGCGTGTACGTGGTTTTATCTGTGTTACTGCGCATCCAACGGGTTGCGA
GGCTAACGTGAAAAAACAGATCGACTACGTTACCACCGAAGGTCCGATTGCGAACGGTCCGAAACGTGTTCTGGTTATTG
GTGCGTCTACCGGTTACGGTCTGGCAGCCCGTATCACGGCGGCTTTTGGTTGCGGCGCAGATACCCTGGGCGTTTTCTTC
GAACGTCCTGGCGAAGAAGGCAAACCGGGTACGTCTGGTTGGTACAATTCTGCGGCGTTCCACAAATTCGCTGCGCAAAA
AGGTCTGTACGCGAAATCTATCAACGGTGATGCGTTCTCTGACGAAATTAAGCAGCTCACCATTGACGCGATCAAACAGG
ACCTCGGTCAGGTTGACCAAGTTATTTACTCTCTCGCGTCTCCACGTCGTACCCACCCGAAGACCGGTGAAGTTTTCAAC
TCTGCGCTGAAACCTATTGGTAATGCTGTAAATCTGCGTGGCCTGGACACCGACAAAGAAGTTATCAAAGAATCTGTCCT
GCAGCCGGCGACCCAGTCTGAAATCGACTCTACCGTTGCGGTCATGGGCGGTGAGGATTGGCAAATGTGGATCGATGCCC
TCCTGGACGCGGGTGTTCTCGCGGAAGGTGCCCAAACCACCGCGTTCACCTACCTGGGTGAGAAAATCACGCACGATATC
TATTGGAACGGTTCTATCGGTGCGGCGAAAAAAGACCTGGACCAGAAAGTTCTGGCGATCCGTGAATCTCTGGCGGCTCA
CGGTGGCGGCGATGCTCGTGTTTCTGTTCTGAAAGCGGTTGTTACCCAGGCGTCTTCTGCGATCCCTATGATGCCGCTCT
ACCTGTCTCTGCTNNTCAAANNTTNTGAAAANNNNAANNNACNCATGANNNTGCATCGANNNGTCTACNCCCNNNNCNAA
NNATTNNNNNNNNNNCNGNNNATNNNNCCNNNNNATGGGANNNNAANNCGTNNNCNNNNNGNNTANNNNNCTNNNNCNNN
NNTCNNANCNGNNCANNANCTNNNNGNNNNNNANNNNANANNNTNNNNNNCNNANNNNTNNNNGNNNNNNANNNNNANNN
NNCNNTNNNNNNNNNNNNNNNGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN

Using PLic Reverse:

NNNNNNNNNNNNGGNNCTCNGTGGTGGTGGTGGTGGTGCTCGAGTGCGGCCGCAAGCTTGTCGACGGAGCTCGAATTCGG
ATCCGTATCCACCTTTACTGTTAACCCTGGATCAGATTCGGGATTTTGACGTCCGGGTTAACATCCGCGTCGTAGTCAAC
ACCGTCGATACCGAAACCGAAGAGGTTGAGGAATTCAGATTTGTAACCCACGAAATCCGTCAGTTGGTAGATGTTGTCGT
TGGTCACCTGGTCCCACAGCTGCTGAACCTGATTCTGGACTTCAGGGTCGAGCTCCTTGTAGTCCGCACGGAGACGACCT
TCTTGGTCCATGTGCGGAGAATCACCGCACAGAGAATCTTTGTAGAGGGAGTAGACCTGCTCGATGCAACCTTCATGCGT
ACCTTTCTCTTTCATAACTTTGAACAGCAGAGACAGGTAGAGCGGCATCATAGGGATCGCAGAAGACGCCTGGGTAACAA
CCGCTTTCAGAACAGAAACACGAGCATCGCCGCCACCGTGAGCCGCCAGAGATTCACGGATCGCCAGAACTTTCTGGTCC
AGGTCTTTTTTCGCCGCACCGATAGAACCGTTCCAATAGATATCGTGCGTGATTTTCTCACCCAGGTAGGTGAACGCGGT
GGTTTGGGCACCTTCCGCGAGAACACCCGCGTCCAGGAGGGCATCGATCCACATTTGCCAATCCTCACCGCCCATGACCG
CAACGGTAGAGTCGATTTCAGACTGGGTCGCCGGCTGCAGGACAGATTCTTTGATAACTTCTTTGTCGGTGTCCAGGCCA
CGCAGATTTACAGCATTACCAATAGGTTTCAGCGCAGAGTTGAAAACTTCACCGGTCTTCGGGTGGGTACGACGTGGAGA
CGCGAGAGAGTAAATAACTTGGTCAACCTGACCGAGGTCCTGTTTGATCGCGTCAATGGTGAGCTGCTTAATTTCGTCAG
ANAACGCATCANCNNNGATAGATTTCGCGTACAGACCNTTTTTGCGCANCGAATTNNNGGNNNNCGCANANNGTACCNAC
CAGANGTTNCCCNGNTTGNNTNNNTCNCCNGGNCNTTCNANAAAACGCCCAGGGNNNNNGCNNCNCANNAANCNNNNGAT
NNGGCNNCNNACGNNNNNNNNANNNNNCNNNNNNAANNNNNTTCGNNCNNNNNNANNNNANNNNNNGGNANNNNNNNANN
NNNTTTNNNNNNTNNCNNNNNNNNNNNNNNNNNNCNNNNNNNNNNNNTAANNNNNNN

(Preexisting) Codon Optimized Sequence:
ATGATTATCAAGCCGCGTGTACGTGGTTTTATCTGTGTTACTGCGCATCCAACGGGTTGCGAGGCTAACGTGAAAAAACAGATCGACTACGTTACCACCGAAGGTCCGATTGCGAACGGTCCGAAACGTGTTCTGGTTATTGGTGCG

BLAST Alignment Results:


Week 6 - 7/8/2013

Transformation and Midi Prep results for FabV. Used 1ml of buffer TE instead of 0.5ml.






Average concentration: 21.5 ng/uL

Submitted to sequencing 7/11/2013 with order number 93094

Week 5 - 7/1/2013

PCR Gel Results (pgbr22)

Lane 1: 100bp DNA Ladder
Lane 2: Sample A
Lane 2: Sample B
Lane 3: Sample C
Lane 4: Sample D (Control, contamination check)

Week 4 - 6/24/2013

RE Digest Gel

1. Skipped
2. 1kb Ladder
3. Uncut Plasmid (Ashlee's)
4. EcoRI (Ashlee's)
5. EcoRI + PvuII (Ashlee's)
6. PVUII (Ashlee's)
7. Uncut Plasmid
8. EcoRI
9. PvuII
10. EcoRI + PvuII

Virtual Gels:

Cut using EcoRI

Cut with PvuII

Cut with EcoRI + PvuII